English: (a,b) NeuN immunostaining in coronal sections containing control (a) or RhebCA (b) expressing neurons. Electroporated neurons are not shown. Scale: 100 μm. (c) NeuN immunostaining (green) of tdTomato+ cells (red) in the RhebCA condition from the white rectangle shown in (b). Scale bar, 100 μm. (d) Myelin basic protein (MBP) immunostaining (green) of tdTomato+ cells (red) in the RhebCA condition illustrating myelin displacement and white matter heterotopia in the corpus callosum (CC). Scale bar, 100 μm. (e) SMI 311 immunostaining in a coronal section containing ipsilateral (RhebCA electroporated) and contralateral ACC. Electroporated neurons are not shown, but strong somatodendritic SMI 311 immunoreactivity is visible in ipsilateral neurons. Scale bar, 100 μm. (f) SMI 311 immunostaining (green) of tdTomato+ cells (red) in the RhebCA (from e) and control conditions. Scale bar, 50 μm. (g,h) Confocal images of control and RhebCA-expressing neurons in layer 2/3 of the ACC. Neurons were microinjected with neurobiotin and post-stained with Alexa Fluor 633 conjugated Streptavidin for visualization. Scale bar, 100 μm. (i) Bar graphs of soma size of control and RhebCA-expressing neurons in layer 2/3. **P<0.01 (Student's t-test). Error bars, s.e.m.