English: Intracellularly stored poly phosphorus (Poly-P) and polyhydroxy-alkanoates (PHA) are the primary targets used to identify phosphorus accumulating organisms (PAOs). The detection of intercellularly stored Poly-P itself is far more reliable than PHA. Polymer staining techniques such as 41,6-diamidino-2-phenylindole (DAPI) are used to identify the presence of PAOs within wastewaters activated sludge. DAPI, a dying molecule, exhibits a maximum fluorescence emission of around 450 nm (blue), when bound to Poly-P, a bathochromic shift in the emission to 525-550 nm occurs, exhibiting a yellowish color. DAPI stains polymeric ions, such as DNA and lipids. DAPI-DNA fluorescence appears blue, whereas both DAPI-Poly-P and DAPI-lipids resonate yellow. To differentiate between the DAPI-poly-P and DAPI-lipids, biomass samples are collected under aerobic conditions where PHA reserves are thought to have been thoroughly consumed. Any yellow dots in this image represent microorganisms that have consumed orthophosphate as a means for binary fission. DAPI-poly-P interaction is extraordinarily precise with the ability to generate an accurate quantum yield from microscopic observation.
نسب العمل إلى مُؤَلِّفه – يلزم نسب العمل إلى مُؤَلِّفه بشكل مناسب وتوفير رابط للرخصة وتحديد ما إذا أجريت تغييرات. بالإمكان القيام بذلك بأية طريقة معقولة، ولكن ليس بأية طريقة تشير إلى أن المرخِّص يوافقك على الاستعمال.
https://creativecommons.org/licenses/by/4.0CC BY 4.0 Creative Commons Attribution 4.0 truetrue